Ayse Kumsal Tekirdag Kosar, PhD

Ayse Kumsal Tekirdag Kosar, PhD

Molecular Cell Biology Scientist

Albert Einstein College of Medicine

Biography

Cell Biologist and Postdoctoral Scientist with over 9 years of research experience on non-coding RNAs and cellular degradation mechanisms (different autophagy pathways) in health and disease states such as Parkinson’s Disease.

My scientific approach is well grounded and detail-oriented, it has matured through my intense PhD and postdoctoral training during which I have published multiple manuscripts, contributed to grants, and mentored several trainees. Furthermore, I have collaborated with numerous international teams and biotech companies during these years.

Some of my strengths are excellence in laboratory and project management, critical thinking, rigorous planning and timely advancement towards measurable outcomes.

I define myself as an intuitive and insightful person who contributes significantly to optimization and improvement of the organization as well as my personal growth.

Interests

  • Autophagy
  • microRNAs
  • Antisense Oligonucleotides
  • Parkinson's Disease

Education

  • PhD in Biological Sciences and Bioengineering, 2015

    Sabanci University

  • BSc in Biological Sciences and Bioengineering, 2010

    Sabanci University

Experience

 
 
 
 
 

Postdoctoral Research Fellow

Albert Einstein College of Medicine

Oct 2015 – Present New York, USA

Mentor: Prof. Ana Maria Cuervo

Investigating Role of Chaperone-Mediated Autophagy in Parkinson's Disease

Responsibilities include:

  • Investigated genetic and chemical modulation of Chaperone-Mediated Autophagy in Parkinson’s Disease mice model as a therapeutic approach to improve age-related proteolysis decline *Significantly contributed on the project to understand lysosomal role of LRRK2 by performing additional lysosomal assays, immunofluorescence and oversee the collaboration on lipidomics analysis
  • Generated a mice colony, carried out extensive behavioral and biochemical characterization that involved learning new techniques of neuroscience field.
  • Mentoring graduate students and providing direct supervision on rotation and research projects
  • Presented data at meetings including yearly funding agency meetings
  • Coordinated various lab activities such as reagent ordering and equipment maintenance
  • Compiled animal behavior protocols and organized lab inventories

Skills:

  • Protein based techniques – Protein extraction and Western Blot
  • Microscopy- Immunoflorescence with Fluorescent microscopy, Confocal microscopy
  • Subcellular and nuclear fractionation, Lysosome isolation, uptake/binding, proteolysis assay
  • Mice colony maintenance, breeding, behavioral analysis.
  • Tissue immunohistochemistry analysis.
  • Brain slice culturing.
  • Survival surgery – Subcutaneous pellet insertion
 
 
 
 
 

Roche Postdoctoral Fellow

Roche

Oct 2015 – Jan 2017 New York

Co-mentors: Prof. Ana Maria Cuervo and Dr. Erich Koller

Investigating role of autophagy in trafficking on antisense oligonucleotides

  • Designed and executed complex in vitro assays including qPCR, ELISA and immunofluorescence techniques to analyze role of modulating different autophagy pathways on trafficking of antisense oligonucleotides
  • Presented data at quarterly progress meetings with Roche Co-mentor
  • Submitted data generated from this project as a manuscript to a prestigious journal of the field and currently under revision

Skills:

  • Mammalian Cell culture, knockdown with siRNA/CRISPR/lentiviral methods
  • Protein based techniques – Protein extraction and Western Blot
  • RNA based techniques- RNA extraction, RT-PCR, qPCR
  • Microscopy- Immunoflorescence with Fluorescent microscopy, Confocal microscopy
  • Operetta High Content Screening - Operation of machine, full comprehension of analysis software